Journal: Frontiers in Microbiology

Article Title: Bacterial Community Associated with the Reef Coral Mussismilia braziliensis's Momentum Boundary Layer over a Diel Cycle

doi: 10.3389/fmicb.2017.00784

Figure Lengend Snippet: Inorganic nutrients, Chlorophyll a and DOC concentrations in M. braziliensis boundary layer and water column over a diel cycle .

Article Snippet: The microbial community associated with M. braziliensis MBL at night was dominated by Proteobacteria (mean of 32.2% of annotated reads), Firmicutes (21.5%), Thaumarchaeota (9.6%), Euryarchaeota (8.4%), Cyanobacteria (5.9%), and Bacteroidetes (4.4%).

Techniques:

Journal: PLoS Currents

Article Title: Weather and Environmental Hazards at Mass Gatherings

doi: 10.1371/4fca9ee30afc4

Figure Lengend Snippet:

Article Snippet: Nov 2005 , College Football, Iowa, USA , Cyclone 15 km from game led to crowd diversion , Mass evacuation No injuries , Public Health Preparedness • Early Warning System • Shelters provided • Flyers given and announcements made • Game able to start following all-clear , Gallus (2006) *Case report.

Techniques: Aerosol, Transmission Assay

Journal: Cell Death & Disease

Article Title: Estrogen affects the negative feedback loop of PTENP1-miR200c to inhibit PTEN expression in the development of endometrioid endometrial carcinoma

doi: 10.1038/s41419-018-1207-4

Figure Lengend Snippet: a In 37 of 40 EEC samples (92.5%), miR-200c was expressed at higher levels than that in adjacent normal tissues. b In 25 of 37 EEC samples (67.6%), PTEN was expressed at lower levels than in adjacent normal tissues. c In 25 of 27 EEC samples (92.6%), PTEN was expressed at lower levels than in adjacent normal tissues. d miR-200c, PTEN, and PTENP1 expression in 40 cases were analyzed, and showed there was a statistically correlation between their expression. e Immunohistochemistry showed that PTEN was lost in most complex atypical hyperplasia and cancer tissues. f GEO database was used to analyze the expression of miR-200c in EEC. g The expression of PTEN in 546 cases of endometrial carcinoma were analyzed in TGCA database by http://ualcan.path.uab.edu/analysis.html . h TGCA database was used to analyze the PTENP1 expression. i – j In four EEC cell lines the expression of PTEN, PTENP1, and miR-200c was detected by qRT-PCR and western blot. There was an opposite relationship between miR-200c and PTENP1/ PTEN. ** P < 0.01

Article Snippet: Four human EEC cell lines (RL-952, Ishikawa, HEC-1B, and JEC) were ordered from ATCC and maintained in RPMI 1640 (Corning, USA) or Dulbecco’s Modified Eagle’s Medium (DMEM; Corning, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 50 μg/ml of penicillin and streptomycin.

Techniques: Expressing, Immunohistochemistry, Quantitative RT-PCR, Western Blot

Journal: Cell Death & Disease

Article Title: Estrogen affects the negative feedback loop of PTENP1-miR200c to inhibit PTEN expression in the development of endometrioid endometrial carcinoma

doi: 10.1038/s41419-018-1207-4

Figure Lengend Snippet: a The expression of estrogen receptor Alpha in four EEC cell lines. b , c The upregulation of miR-200c and downregulation of PTEN protein were disappeared after E2-induced for 48 h when stable knockdown the ESR1 in RL-952 cell lines. d , e The expression of miR-200c was increased and PTEN protein was downregulated after E2-induced for 48 h when stable overexpression the ESR1 in JEC cell lines. All results are presented as the mean of triplicate assays, with S.D. ** P < 0.01

Article Snippet: Four human EEC cell lines (RL-952, Ishikawa, HEC-1B, and JEC) were ordered from ATCC and maintained in RPMI 1640 (Corning, USA) or Dulbecco’s Modified Eagle’s Medium (DMEM; Corning, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 50 μg/ml of penicillin and streptomycin.

Techniques: Expressing, Knockdown, Over Expression

Journal: Cell Death & Disease

Article Title: Estrogen affects the negative feedback loop of PTENP1-miR200c to inhibit PTEN expression in the development of endometrioid endometrial carcinoma

doi: 10.1038/s41419-018-1207-4

Figure Lengend Snippet: E2 activated the PI3K-AKT-mTOR pathway by upregulating miR-200c expression in EEC cells. Western blot showed that phosphorylated AKT and PI3K protein could be upregulated or downregulated in RL-952 cells by tranfecting miR-200c mimic or inhibitor combine with E2-induced 48 h

Article Snippet: Four human EEC cell lines (RL-952, Ishikawa, HEC-1B, and JEC) were ordered from ATCC and maintained in RPMI 1640 (Corning, USA) or Dulbecco’s Modified Eagle’s Medium (DMEM; Corning, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 50 μg/ml of penicillin and streptomycin.

Techniques: Expressing, Western Blot